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windows server 2012 iis8 app pool permission issue

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Need help in IIS8 application pool, as i am getting an error message after creating and allocated to site, the same demo site is working in default app pool but not in newly created apppool.

Server Error in 'Demo' Application.
Required permissions cannot be acquired.
Description: An unhandled exception occurred during the execution of the current web request. Please review the stack trace for more information about the error and where it originated in the code.

Exception Details: System.Security.Policy.PolicyException: Required permissions cannot be acquired.

Source Error:

An unhandled exception was generated during the execution of the current web request. Information regarding the origin and location of the exception can be identified using the exception stack trace below.

new app pool has been created under .net version 2.0

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